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invivo mab anti mouse cd40  (Bio X Cell)


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    Bio X Cell invivo mab anti mouse cd40
    ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse <t>anti-CD40</t> (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.
    Invivo Mab Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invivo+mab+anti+mouse+cd40/InVivoMAb+anti-mouse+CD40/pmc12915712-398-0-4
    Average 96 stars, based on 204 article reviews
    invivo mab anti mouse cd40 - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury"

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury

    Journal: Nature immunology

    doi: 10.1038/s41590-025-02359-5

    ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.
    Figure Legend Snippet: ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.

    Techniques Used: Adoptive Transfer Assay, Single Cell, RNA Sequencing, Gene Expression, Expressing, Flow Cytometry, Ex Vivo, MANN-WHITNEY

    (a) Gating strategy for B cells. (b) Representative plots of LTβ + CD19 + B220 + B cells from naive SJL/J splenocytes either unstimulated or stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) ex vivo after pre-treatment with BTKi (10nM) or equivalent Vol of culture medium for 1 hour.
    Figure Legend Snippet: (a) Gating strategy for B cells. (b) Representative plots of LTβ + CD19 + B220 + B cells from naive SJL/J splenocytes either unstimulated or stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) ex vivo after pre-treatment with BTKi (10nM) or equivalent Vol of culture medium for 1 hour.

    Techniques Used: Ex Vivo

    Related Articles

    Adoptive Transfer Assay:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    Single Cell:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    RNA Sequencing:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    Gene Expression:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    Expressing:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    Flow Cytometry:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    Ex Vivo:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.

    MANN-WHITNEY:

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury
    Article Snippet: Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.Cells were placed in the incubator at 37°C 5%CO2 for 1 hour to rest, then BTK inhibitor (or culture medium) was added at a concentration of 10nM for 1 hour.. InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).. Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.Cells were harvested, washed with PBS and stained with Live/Dead Fixable Aqua (Invitrogen) at a dilution of 1:1000 for 20 min on ice.



    Similar Products

    invivo mab anti mouse cd40  (Bio X Cell)
    96
    Bio X Cell invivo mab anti mouse cd40
    ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse <t>anti-CD40</t> (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.
    Invivo Mab Anti Mouse Cd40, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invivo+mab+anti+mouse+cd40/InVivoMAb+anti-mouse+CD40/pmc12915712-398-0-4
    Average 96 stars, based on 1 article reviews
    invivo mab anti mouse cd40 - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    anti mouse cd40 invivo mab  (Bio X Cell)
    96
    Bio X Cell anti mouse cd40 invivo mab
    ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse <t>anti-CD40</t> (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.
    Anti Mouse Cd40 Invivo Mab, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invivo+mab+anti+mouse+cd40/InVivoMAb+anti-mouse+CD40/10__1080_slash_2162402x__2017__1328340-71-1-6
    Average 96 stars, based on 1 article reviews
    anti mouse cd40 invivo mab - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.

    Journal: Nature immunology

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury

    doi: 10.1038/s41590-025-02359-5

    Figure Lengend Snippet: ( a ) qPCR of Lta and Ltb in the leptomeninges of vehicle–treated or BTKi–treated SJL/J adoptive transfer EAE mice at peak disease. ( b-d ) Single-cell RNA sequencing of leptomeninges from young (n=2), and old (n=2) SJL/J mice at acute A/T EAE and appropriate age-matched, naïve controls young and old EAE mice. Data shown represent 2 biological and experimental repeats, each with an n of 1 for each group analyzed. ( b ) Violin plot showing relative gene expression of Btk and lymphotoxin ligand genes ( Lta , Ltb ) in identified cell clusters stratified by age. ( c ) Uniform manifold projection (UMAP) of 16,568 leptomeningeal cells after unsupervised clustering. ( d ) Gene co-expression of Btk with Ltb, Lta projected on the global UMAP. ( e ) Flow cytometry of the LTβ positive population in CD19+ B220+ B cells from naive SJL/J (n=7) or Ltb −/− mice (n=7) splenocytes stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) (Stim) ex vivo or pre-treated 1 hour with BTKi (10nM). Data in ( a ) and ( e ) are shown as means ± SD. Statistical analysis was conducted using two-sided unpaired t test for (a) and two-sided Mann-Whitney for (e). ns, not significant. n = 7 in each group.

    Article Snippet: InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).

    Techniques: Adoptive Transfer Assay, Single Cell, RNA Sequencing, Gene Expression, Expressing, Flow Cytometry, Ex Vivo, MANN-WHITNEY

    (a) Gating strategy for B cells. (b) Representative plots of LTβ + CD19 + B220 + B cells from naive SJL/J splenocytes either unstimulated or stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) ex vivo after pre-treatment with BTKi (10nM) or equivalent Vol of culture medium for 1 hour.

    Journal: Nature immunology

    Article Title: Lymphotoxin-dependent elevated meningeal CXCL13:BAFF ratios drive grey matter injury

    doi: 10.1038/s41590-025-02359-5

    Figure Lengend Snippet: (a) Gating strategy for B cells. (b) Representative plots of LTβ + CD19 + B220 + B cells from naive SJL/J splenocytes either unstimulated or stimulated with mouse anti-CD40 (5ug/ml) + LPS (1ug/ml) ex vivo after pre-treatment with BTKi (10nM) or equivalent Vol of culture medium for 1 hour.

    Article Snippet: InVivo MAb anti-mouse CD40 (BioxCell BE0016–2) (5ug/ml) + LPS (Sigma-Aldrich L2880) (1ug/ml) were added to the culture overnight (18–20 h).

    Techniques: Ex Vivo